Molecular cloning of a gene encoding an arabinogalactan-protein from pear (Pyrus communis) cell suspension culture.
نویسندگان
چکیده
منابع مشابه
Structure of the glycosylphosphatidylinositol anchor of an arabinogalactan protein from Pyrus communis suspension-cultured cells.
Arabinogalactan proteins (AGPs) are proteoglycans of higher plants, which are implicated in growth and development. We recently have shown that two AGPs, NaAGP1 (from Nicotiana alata styles) and PcAGP1 (from Pyrus communis cell suspension culture), are modified by the addition of a glycosylphosphatidylinositol (GPI) anchor. However, paradoxically, both AGPs were buffer soluble rather than membr...
متن کاملProtein Synthesis and Breakdown during Heat Shock of Cultured Pear ( Pyrus communis 1 . ) Cells
Cultured pear (Pyrus communis 1. cv Passe Crassane) cells were subjected to temperatures of 39,42, and 45°C. Heat-shock protein (hsp) synthesis was greater at 39'C than at temperatures above 40'C and continued for up to 8 h. Both cellular uptake of radiolabeled methionine and total protein synthesis were progressively lower as the temperature was increased. Polysome levels decreased immediately...
متن کاملCloning and molecular characterization of TaERF6, a gene encoding a bread wheat ethylene response factor
Ethylene response factor proteins are important for regulating gene expression under different stresses. Different isoforms for ERF have previously isolated from bread wheat (Triticum aestivum L.) and related genera and called from TaERF1 to TaERF5. We isolated, cloned and molecular characterized a novel one based on TdERF1, an isoform in durum wheat (Tri...
متن کاملProtein Synthesis and Breakdown during Heat Shock of Cultured Pear (Pyrus communis L.) Cells.
Cultured pear (Pyrus communis L. cv Passe Crassane) cells were subjected to temperatures of 39, 42, and 45[deg]C. Heat-shock protein (hsp) synthesis was greater at 30[deg]C than at temperatures above 40[deg]C and continued for up to 8 h. Both cellular uptake of radiolabeled methionine and total protein synthesis were progressively lower as the temperature was increased. Polysome levels decrease...
متن کاملProtein Synthesis and Breakdown during Heat Shock of Cultured Pear ( Pyrus communis 1 . ) Cells lan
Cultured pear (Pyrus communis 1. cv Passe Crassane) cells were subjected to temperatures of 39,42, and 45°C. Heat-shock protein (hsp) synthesis was greater at 39'C than at temperatures above 40'C and continued for up to 8 h. Both cellular uptake of radiolabeled methionine and total protein synthesis were progressively lower as the temperature was increased. Polysome levels decreased immediately...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1994
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.91.22.10305